ABSTRACT
Background and Aims: Chromosome abnormalities are a major cause of miscarriage and neonatal mortality. The present study aimed to determine the association of fetal and parents chromosomal abnormalities with congenital anomalies
Methods: A cross-sectional study was performed in a tertiary referral center [Afzalipour Hospital] over 16 months period [2011-2012]. The study groups consisted of 77 fetuses over 14 weeks and their parents. Fetuses had apparent anomaly after abortion or birth, or showed a defect organs in targeted sonographic examination. DNA extractions were from fetus tissues for investigation of chromosome abnormalities using [multiplex ligation-dependent probe amplification] MLPA. Cytogenetic analysis for parents was performed with G-banding technique. Eventually data were analyzed by statistical software SPSS using t-test, chisquare and logistic regression
Results: Karyotyping of fetus was 46xx in 27 [35.9%] and 46xy in 25 [32.1%] cases. Twenty-five fetuses had chromosomal abnormalities. The common chromosomal abnormalities were multiple deletion and duplication on different chromosomes in 4 [6.5%] and Down syndrome in 3 [3.9%] of them. This study showed a statistically significant association between the extremity anomalies [P=0.0070], oligohydroamnious [P=0.0050], ascitis [P=0.0001], increased nuchal translucency [P=0.0001], esophageal atresia [P=0.0070], duodenal atresia [P=0.0001], polycystic kidney [P=0.0070], echogenic bowel [P=0.0001], plural effusion [P=0.0001] and cardiomegaly [P=0.0010]. There were no statistically significant association between the chromosomal abnormalities in fetus and parents [P=0.5700]
Conclusion: In our study, there was no association between the chromosomal abnormalities in fetus and parents. It can be concluded that many chromosomal defects occur during the formation of sperm or ovum. Detection of major congenital malformations should draw attention to the possibility of a chromosomal disorder in fetus. Therefore, MLPA is a low cost method for detecting a wide range of the most common chromosomal disorders in a short time
ABSTRACT
Maternal infections with parvovirus B19 and cytomegalovirus [CMV] maybe associated with intrauterine fetal death. The aim of this study was to compare frequency of CMV and Parvovirus B19 Infections in intrauterine fetal death [IUFD] and normal pregnancy. In a case-control study in Afzalipour Hospital during 2006 placental biopsies were collected from 70 cases of IUFD and 70 normal term pregnancies as controls and were examined for CMV DNA and parvovirus B19 DNA using polymerase chain reaction [PCR]. Maternal viral serology was measured as well. Cytomegalovirus DNA on placental biopsies were recovered in 44.3% [31 cases] of cases and 5.7% [4cases] of the controls. [OR = 11.6, 95% CI 4.2-32.3, P = 0.0001]. CMV IgG antibodies were found in 98.6% of two groups. In whole, 44.3% of case group and 5.7% of the control group had CMV IgM antibodies [OR = 13.11, 95% CI 4.3-39.95, P = 0.0001]. Parvovirus DNA were found in 10% [7 cases] of case group and 1.4% [lease] of the control group [OR = 7.7, 95% CI 0.92-64, P = 0.06]. 37.2% of cases and 22.1% of the controls had IgG. IgM antibodies were found in 10% [7 cases] of the case group and 2.9% [2 cases] of the control group [OR = 3.78, 95%CI 0.76-18.9, P = 0.16]. CMV maybe considered as an etiologic factor in fetal death. PCR on placental and presence of IgM antibodies can be used for diagnosis of this infection. Association of maternal parvovirus B19 infection with IUFD is not clear